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Different criteria have been used to pick predicted peptides. In our case, along with a binding threshold for all mutated peptides, we distinguished two teams regarding the binding capacity of the WT peptide. In the primary group , the place WT peptides had been poor or null binders, an important proportion of peptides contained the mutation at HLA-anchoring residues (e.g. peptides CAM69-6, CAM73-9). However, in the second group all peptides contained the mutation at non-anchor residues.

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For those patients with out there RNAseq, quartiles of FPKM had been calculated. Mutations identified by WES evaluation were considered as “expressed mutations” if at least one of many gene counter algorithms was expressed in 3rd or 4th quartile. Once mutations have been filtered, 29-mer amino acid sequences, containing the mutated residue at position 15, were designed.

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